permeabilize

the protocol requires you to permeabilize the cell membrane before adding the antibodies.

researchers often permeabilize cells with detergent to allow antibody penetration.

we need to permeabilize the tissue samples to detect intracellular proteins.

gentle treatment is necessary to permeabilize bacterial cells without destroying them.

the cytoplasmic membrane can be permeabilized using mild surfactants.

to permeabilize the plasma membrane, incubate cells with 0.1% triton x-100.

fixed cells must be permeabilized carefully to preserve morphology.

scientists permeabilize samples for immunostaining to visualize target proteins.

after permeabilization, we can proceed to stain the cells with fluorescent markers.

a brief incubation with saponin can effectively permeabilize cellular membranes.

over-permeabilization may damage cells and affect experimental results.

proper permeabilization enables antibodies to access intracellular epitopes.